Rolf Günther, Andreas Schober, Andreas Schweinhorst, Peter Stadler
Paper #: 95-10-090
Synthesis of random libraries of polynucleotide sequences has become a standard procedure of biotechnology; in combination with amplification methods such as the polymerase chain reaction (PCR) [1] this allows for selection experiments in which large pools of sequences are screened for a desired function. Provided the desired function can be used to design a selection constraint, these basic techniques can be combined in elegant in vitro experiments consisting of alternate cycles of selection and amplification of the selected function.